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Pasteur Veterinary Laboratory

Veterinary laboratories are a useful aid in diseases control for veterinarians and producers. The main function of these laboratories is to provide assistance for veterinarian in disease diagnosis.
The Pasteur veterinary laboratory consists of several sections, including molecular biology, serology, microbiology (bacteriology, mycology and parasitology) and pathology. All of these sections are located in the central laboratory building in Parcham Ave., Tehran.
In this laboratory, veterinarians with the cooperation of experienced laboratory technicians have the responsibility for several steps of test performance including: sample reception, form completion, investigation of veterinarian request, test operation and report preparations.
All of the tests and reports preparations are done by modern facilities, standard kits and computer systems.
The main activities in this laboratory center are as follows:

1 – Molecular Diagnosis and Finding of Pathogen by PCR
Polymerase chain reaction (PCR) is a modern and precise system for pathogen identification. The basis of this method is creating copies of specific fragments of DNA for diagnoing the microorganisms.
The PCR application is very common in different sciences. For example, small samples of DNA, such as those found in a strand of hair at a crime scene, can produce sufficient copies to carry out forensic tests.
In veterinary sciences the PCR has widely uses for finding and identification of several pathogens including Mycoplasma agents in poultry flocks such as MG and MS, diagnosis and identification of different viruses including Avian Influenza, Infection Bronchitis etc.
By the way, this method is a very accurate test for aquaculture diseases diagnosis.
Pasteur veterinary laboratory is proud that by using expert poultry veterinarian and experienced technician in laboratory sciences and application of modern facilities and standard international kits, can offer complete services for disease finding and diagnosis to Iran’s veterinarians and animal farmers.
By considering this facts that PCR sampling needs special procedure, please contact Pasteur laboratory for more information about sampling.

 

2- Biochemical Analysis of Blood Samples
Determination of blood biochemical profile is a very useful and rapid test, as it gives the veterinarian an overview of how your animal body functions. Usually veterinarians by interpretation of these profiles can find internall disorders and/or where dysfunction may be occurring.
It is better to send the animal to laboratory for blood sampling. If it is impossible, depend on the requested test, a blood sample is sent to a laboratory with or without anticoagulant.
The tests such as blood sugar, urea, albumen, total protein, immunoglobulin, IgG, bilirubin, Ca, P, Cl, Cholesterol, Kereatinin, Mg, Mn, nitrate, K, Na, ureic acid, enzymes(Ck, GGT, Alkp, Ast), Differential Counts(CBC), PCV are routine in Pasteur veterinary laboratory.
Interpretation of test results depends on animal species, size, sex and disease and must be carried out by a veterinarian. Changes in the biochemical profile can help a doctor to diagnose diseases affecting the liver, kidney, pancreas, or endocrine systems. Once diagnosed, many of these diseases are treatable.
In most cases, especially in pet animals, a biochemical profile may be recommended in intervals, as part of a health maintenance plan and disease control.
We cordially request from dear veterinarians, industrial farmers and pet animal owners, for more information about biochemical test and directions for interpretation of test results, contact us.

 

3- Serological Tests for Poultry Blood & Egg
Serological tests are sometimes regarded as the principal means of evaluating infectious disease in poultry. Serological methods, however, have been applied widely in the monitoring of vaccination response in poultry.
The several tests including IF, AGID, RST, HI, EISA, for Newcastle disease diagnosis, Influenza, Infectious Bursal Disease, Infectious Bronchitis, Laryngoterachitis, Reovirus, Encephalomyelitis, MG, MS, Ornitobacter, Pasteurelosis, Salmonelosis, Anemia, Leucosis-J and TRT in chicken, turkey, quail, duck, ostrich, pigeon and caged birds, are the main activities of Pasteur veterinary laboratory.
The first report of the use of a serological test in the diagnosis and control of a disease of poultry was made in early 20th century by using of a Tube Agglutination Test for Pullorum disease. Rapid Agglutination Tests are still widely performed for Mycoplasma gallisepticum, and M. synoviae in chickens.
The blood sample that will be sent to laboratory must be taken before bird feeding (as they are fasted).Do not freeze blood or serum, and please send samples to laboratory as soon as possible (less than 24 hours).

Haemagglutination Inhibition (HI) is another type of serological test. Its application is not only for detection but also for quantification of specific antibody against Newcastle, Influenza, EDS, Infectious Bronchitis and MG in poultry.
Enzyme Linked Immune Sorbent Assay (Elisa) is one of the serological testing method to be widely taken up by the poultry industry. Initially, the Elisa technique used by scientists worked in university and government laboratories. However, producing commercially kits, allowed relatively simple laboratories to carry out a range of serological tests for important poultry pathogens.
Just as mammals pass on a package of specific antibodies to their progeny via the first milk or colostrums, birds achieve the same result by secreting a large amount of antibody into the yolk of their eggs. For this reason yolk extracts can be used as a substitute for serum samples for serological monitoring.
Pasteur veterinary laboratory synchronous with modern scientific and research centers, by updating test methods and technology transfer, especially for diagnosis in poultry diseases field, will do it’s best for complete and precise services for it’s clients.
Also for research purposes and special requests, many other tests such as serum-neutralization, immunofluorescence, complement fixation and so on, is well established in Pasteur veterinary laboratory.

 

4- Culture and Antibiogram
Culture is propagation of bacteria cells in a sample for identification and if necessary antibiogram test. The main bacteria that are cultured and identified by Pasteur laboratory are: Mycoplasma, Staphylococcus, Streptococcus, Listeria, Entrobacteriacea, Campylobacter, Corynebacteria, Salmonella, Vibrio, Haemophilous, Pasteurella, Pseudomonas, Morexalla, Niesseria, Brucella, Bacillus, clostridium, Aeromonus, Lactobacillus.
Antibiogram test, measures the ability of an antibiotic or other antimicrobial agent to inhibit bacterial growth in vitro. This ability may be estimated by either the dilution method or the diffusion method. Antibiotic susceptibility testing has become a very essential step for the proper treatment of infectious diseases.
It is used to guide the veterinarian in selecting the best antimicrobial agent and also to accumulate epidemiological information on the resistance of microorganisms of public health importance.
In Pasteur veterinary laboratory, the choice of drugs used in a routine antibiogram are divided into two sets:
1- Set 1 includes drugs that are available in most veterinary pharmacy in Iran.
2- Set 2 are to be performed only at the special request of the veterinarian, or when the causative organism is resistant to the first-choice drugs.
For more information about culture and antibiogram tests, please do not hesitate to contact us.

 

5- Histopathology
Histopathology is one of the major tools of diagnosis in veterinary. The major advantages of histopathology are the ability to provide a precise diagnosis of some diseases, as well as demonstrating the tissue reactions.
Furthermore, veterinary histopathology is the only way to diagnose some diseases. As a general term, Histopathology is a field of pathology which specializes in the histological study of diseased tissue.
Histopathological examination of tissues starts with surgery, biopsy or autopsy. The tissue is removed, and then placed in a fixative which stabilizes the tissues to prevent decay. The most common fixative is formalin (10% formaldehyde in water).
For formalin 10% preparation, please mix one part of commercial formalin (for example 100 ml) with nine parts of water (for example 900 ml), and use this solution for primary preservation of tissue.
In Pasteur laboratory, we provide commonly used histopathological methods for veterinary diagnosis.
For more histopathology information, other than those mentioned above or
for more details about sampling and tissue preservation, please contact us.

6-Serological Tests on Livestock Blood and Milk
Serological tests on blood and milk are the common tests for health determination and diseases diagnosis in animals including cattle, sheep, gout, horse and dogs.
Pasteur laboratory by performing serological tests, particularly for viral diseases in large and pet animals, has special services for veterinarian and animal owners.
Different diseases including Leucosis, Bovine Viral Diarrhea, Mucosal Diseases, infectious bovine rhinotracheitis, Pestivirus, Salmonella, Listeria, Chlamydia, Mycoplasma and Bovine Johne`s Diseases, are identified and diagnosed by several methods in this laboratory.
Also pregnancy diagnosis by determination of blood progesterone level and achievement of all of the tests related to the milk are other activities of Pasteur veterinary laboratory.
For more information about serological tests on blood and milk, please contact Pasteur veterinary laboratory.

 

Complete Tests List
Abbreviation list