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Veterinary laboratories
are a useful aid in
diseases control for
veterinarians and
producers. The main
function of these
laboratories is to
provide assistance for
veterinarian in disease
diagnosis.
The Pasteur veterinary
laboratory consists of
several sections,
including molecular
biology, serology,
microbiology
(bacteriology, mycology
and parasitology) and
pathology. All of these
sections are located in
the central laboratory
building in Parcham
Ave., Tehran.
In this laboratory,
veterinarians with the
cooperation of
experienced laboratory
technicians have the
responsibility for
several steps of test
performance including:
sample reception, form
completion,
investigation of
veterinarian request,
test operation and
report preparations.
All of the tests and
reports preparations are
done by modern
facilities, standard
kits and computer
systems.
The main activities in
this laboratory center
are as follows:
1 –
Molecular Diagnosis and
Finding of Pathogen by
PCR
Polymerase chain
reaction (PCR) is a
modern and precise
system for pathogen
identification. The
basis of this method is
creating copies of
specific fragments of
DNA for diagnoing the
microorganisms.
The PCR application is
very common in different
sciences. For example,
small samples of DNA,
such as those found in a
strand of hair at a
crime scene, can produce
sufficient copies to
carry out forensic
tests.
In veterinary sciences
the PCR has widely uses
for finding and
identification of
several pathogens
including Mycoplasma
agents in poultry flocks
such as MG and MS,
diagnosis and
identification of
different viruses
including Avian
Influenza, Infection
Bronchitis etc.
By the way, this method
is a very accurate test
for aquaculture diseases
diagnosis.
Pasteur
veterinary laboratory is
proud that by using
expert poultry
veterinarian and
experienced technician
in laboratory sciences
and application of
modern facilities and
standard international
kits, can offer complete
services for disease
finding and diagnosis to
Iran’s veterinarians and
animal farmers.
By considering this
facts that PCR sampling
needs special procedure,
please contact
Pasteur laboratory
for more information
about sampling.
2- Biochemical
Analysis
of Blood Samples
Determination of blood
biochemical profile is a
very useful and rapid
test, as it gives the
veterinarian an overview
of how your animal body
functions. Usually
veterinarians by
interpretation of these
profiles can find
internall disorders
and/or where dysfunction
may be occurring.
It is better to send
the animal to laboratory
for blood sampling. If
it is impossible, depend
on the requested test, a
blood sample is sent to
a laboratory with or
without anticoagulant.
The tests such as blood
sugar, urea, albumen,
total protein,
immunoglobulin, IgG,
bilirubin, Ca, P, Cl,
Cholesterol, Kereatinin,
Mg, Mn, nitrate, K, Na,
ureic acid, enzymes(Ck,
GGT, Alkp, Ast),
Differential
Counts(CBC), PCV are
routine in Pasteur
veterinary laboratory.
Interpretation of test
results depends on
animal species, size,
sex and disease and must
be carried out by a
veterinarian. Changes in
the biochemical profile
can help a doctor to
diagnose diseases
affecting the liver,
kidney, pancreas, or
endocrine systems. Once
diagnosed, many of these
diseases are treatable.
In most cases,
especially in pet
animals, a biochemical
profile may be
recommended in
intervals, as part of a
health maintenance plan
and disease control.
We cordially request
from dear veterinarians,
industrial farmers and
pet animal owners, for
more information about
biochemical test and
directions for
interpretation of test
results,
contact us.
3-
Serological Tests for
Poultry Blood & Egg
Serological tests are
sometimes regarded as
the principal means of
evaluating infectious
disease in poultry.
Serological methods,
however, have been
applied widely in the
monitoring of
vaccination response in
poultry.
The several tests
including IF, AGID, RST,
HI, EISA, for Newcastle
disease diagnosis,
Influenza, Infectious
Bursal Disease,
Infectious Bronchitis,
Laryngoterachitis,
Reovirus,
Encephalomyelitis, MG,
MS, Ornitobacter,
Pasteurelosis,
Salmonelosis, Anemia,
Leucosis-J and TRT in
chicken, turkey, quail,
duck, ostrich, pigeon
and caged birds, are the
main activities of
Pasteur veterinary
laboratory.
The first report of the
use of a serological
test in the diagnosis
and control of a disease
of poultry was made in
early 20th century by
using of a Tube
Agglutination Test
for Pullorum disease.
Rapid Agglutination
Tests are still widely
performed for
Mycoplasma
gallisepticum, and
M. synoviae in
chickens.
The blood sample that
will be sent to
laboratory must be taken
before bird feeding (as
they are fasted).Do not
freeze blood or serum,
and please send samples
to laboratory as soon as
possible (less than 24
hours).
Haemagglutination
Inhibition (HI) is
another type of
serological test. Its
application is not only
for detection but also
for quantification of
specific antibody
against Newcastle,
Influenza, EDS,
Infectious Bronchitis
and MG in poultry.
Enzyme Linked Immune
Sorbent Assay (Elisa) is
one of the serological
testing method to be
widely taken up by the
poultry industry.
Initially, the Elisa
technique used by
scientists worked in
university and
government laboratories.
However, producing
commercially kits,
allowed relatively
simple laboratories to
carry out a range of
serological tests for
important poultry
pathogens.
Just as mammals pass on
a package of specific
antibodies to their
progeny via the first
milk or colostrums,
birds achieve the same
result by secreting a
large amount of antibody
into the yolk of their
eggs. For this reason
yolk extracts can be
used as a substitute for
serum samples for
serological monitoring.
Pasteur veterinary
laboratory synchronous
with modern scientific
and research centers, by
updating test methods
and technology transfer,
especially for diagnosis
in poultry diseases
field, will do it’s best
for complete and precise
services for it’s
clients.
Also for research
purposes and special
requests, many other
tests such as
serum-neutralization,
immunofluorescence,
complement fixation and
so on, is well
established in Pasteur
veterinary laboratory.
4- Culture and
Antibiogram
Culture is
propagation of bacteria
cells in a sample for
identification and if
necessary antibiogram
test. The main bacteria
that are cultured and
identified by Pasteur
laboratory are:
Mycoplasma,
Staphylococcus,
Streptococcus, Listeria,
Entrobacteriacea,
Campylobacter,
Corynebacteria,
Salmonella, Vibrio,
Haemophilous,
Pasteurella,
Pseudomonas, Morexalla,
Niesseria, Brucella,
Bacillus, clostridium,
Aeromonus,
Lactobacillus.
Antibiogram test,
measures the ability of
an antibiotic or other
antimicrobial agent to
inhibit bacterial growth
in vitro. This ability
may be estimated by
either the dilution
method or the diffusion
method. Antibiotic
susceptibility testing
has become a very
essential step for the
proper treatment of
infectious diseases.
It is used to guide the
veterinarian in
selecting the best
antimicrobial agent and
also to accumulate
epidemiological
information on the
resistance of
microorganisms of public
health importance.
In Pasteur
veterinary laboratory,
the choice of drugs used
in a routine antibiogram
are divided into two
sets:
1- Set 1 includes drugs
that are available in
most veterinary pharmacy
in Iran.
2- Set 2 are to be
performed only at the
special request of the
veterinarian, or when
the causative organism
is resistant to the
first-choice drugs.
For more information
about culture and
antibiogram tests,
please do not hesitate
to contact us.
 5-
Histopathology
Histopathology is one of
the major tools of diagnosis in veterinary. The major advantages of
histopathology are the ability to provide a precise diagnosis of some diseases,
as well as demonstrating the tissue reactions.
Furthermore, veterinary histopathology is the only way to diagnose some
diseases. As a general term, Histopathology is a field of pathology which
specializes in the histological study of diseased tissue.
Histopathological examination of tissues starts with surgery, biopsy or autopsy.
The tissue is removed, and then placed in a fixative which stabilizes the
tissues to prevent decay. The most common fixative is formalin (10% formaldehyde
in water).
For formalin 10% preparation, please mix one part of commercial formalin (for
example 100 ml) with nine parts of water (for example 900 ml), and use this
solution for primary preservation of tissue.
In Pasteur laboratory, we provide commonly used histopathological methods
for veterinary diagnosis.
For more histopathology information, other than those mentioned above or
for more details about sampling and tissue preservation,
please contact us. 
6-Serological Tests on
Livestock Blood and Milk
Serological tests on
blood and milk are the
common tests for health
determination and
diseases diagnosis in
animals including
cattle, sheep, gout,
horse and dogs.
Pasteur
laboratory by performing
serological tests,
particularly for viral
diseases in large and
pet animals, has special
services for
veterinarian and animal
owners.
Different diseases
including Leucosis,
Bovine Viral Diarrhea,
Mucosal Diseases,
infectious bovine
rhinotracheitis,
Pestivirus, Salmonella,
Listeria, Chlamydia,
Mycoplasma and Bovine
Johne`s Diseases, are
identified and diagnosed
by several methods in
this laboratory.
Also pregnancy diagnosis
by determination of
blood progesterone level
and achievement of all
of the tests related to
the milk are other
activities of Pasteur
veterinary laboratory.
For more information
about serological tests
on blood and milk,
please contact
Pasteur veterinary
laboratory.
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